Table 2 Primer sequences, amplicon lengths and efficiency for qPCR assays
Gene description and purpose | Primer sequence (forward / reverse) | Amplicon length (bp) | qPCRa Efficiency |
|---|---|---|---|
VvHyd2 PCR | CAAGTATCTGGGCGAAAACC / CATTCTGGGGAAGAGCAAA | 509 | – |
VvHyd2 qPCR | CATCATAGGCGTCATCTTCG / GGACTCTTGCTCTTCCGTGA | 118 | 1.93 |
VvUGT PCR | TAAATACGTTTATTGAGCTTGA / ACACCGTACCATATGCTTTC | 468 | – |
VvUGT qPCR | TCGCTTCTTGTGGTCCCTTC / CCAATTCTAGCCGTCCGATG | 120 | 1.94 |
VvBG2 PCR | TCTGGCACCTCTGCTTATCA / CCCTGTTGTGTTTCCTGATACT | 644 | – |
VvBG2 qPCR | TTTGCTTTGGGTGGTTATGA / GCCAACAAGATGTGATGTGC | 125 | 1.93 |
VvNCED1 qPCR | CCTCTGTCTCACAGCAATGGAACT / ATTACCGGCGATTTGCACTCT | 140 | 1.96 |