Fig. 6

Relative expression profiles of ABA metabolism genes (a, VvNCED1; b, VvHyd2; c, VvUGT; and d, VvBG2) in grapevine cv. Mencía somatic embryo aggregates cultured for 5 weeks on DM1 differentiation medium supplemented with 6% sucrose or over a semipermeable membrane on the same DM1 medium with 6% sucrose. Somatic embryo aggregates collected just at the time of transfer to DM1 medium (nontreated samples) were used as the calibration group for the relative expression analyses. GAPDH(m) and EF1-α(m) were used as the reference genes for normalization. Data are represented as the means (± standard error) of two independent experiments. Asterisks indicate statistically significant differences between the calibrator group and the analyzed group, whereas arrows indicate statistically significant differences between treatments with or without the membrane, which in both cases were calculated using REST-2009© software (p < 0.05)