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Fig. 2 | BMC Plant Biology

Fig. 2

From: BrrICE1.1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of turnip (Brassica rapa var. rapa)

Fig. 2

Transcriptome and qRT-PCR analysis of key genes involved in the polyamine biosynthesis pathway in turnip. a Principal component analysis (PCA) of transcriptome data. The scale of the axis is the relative distance. Different colors or shapes represent different groups of samples under freezing treatments. b Different trend (subcluster_1) analysis of DEGs. c Scatterplot of KEGG pathways enriched for differentially expressed genes (DEGs) in subcluster_1. The top 5 enriched pathway terms in the KEGG database are listed. d Polyamine biosynthetic pathway (right) in plants. e qPCR analysis of key gene in polyamines biosynthesis, with three biological and technical replicates. The data are analyzed by one-way ANOVA (Tukey’s test). Different letters indicate significant difference (P < 0.05). CK, C1, C4, C6, F2, F4, and R12 represent the samples from the untreated control; 4 °C at 1 h, 3 h, and 6 h; − 2 °C at 2 h; − 4 °C 1 h; and recovery for 12 h, respectively

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BMC Plant Biology

ISSN: 1471-2229

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