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Fig. 9 | BMC Plant Biology

Fig. 9

From: The Nicotiana tabacum L. major latex protein-like protein 423 (NtMLP423) positively regulates drought tolerance by ABA-dependent pathway

Fig. 9

NtWRKY71 binds to the NtMLP423 promoter (A) NtWRKY71 regulates the activity of the NtMLP423 promoter. a: 62SK + pNtMLP423-LUC was used as the reference; b: NtWRKY71-62SK + pNtMLP423-LUC. The vectors were infiltrated into tobacco leaves by Agrobacterium tumefaciens GV3101 and then observed by firefly luciferase complementation imaging. Detection of LUC/REN activity verified that NtWRKY71 activated the NtMLP423 promoter. Data points represent means ± SE (n = 3). * indicated significant difference (P < 0.05). b Yeast one-hybrid analysis showing that NtWRKY71 was bound to W-box in the NtMLP423 promoter. c EMSA showing that the NtWRKY71 fusion protein was directly bound to the NtMLP423 promoter on W-box in vitro

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BMC Plant Biology

ISSN: 1471-2229

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