Fig. 6From: iTRAQ-based comparative proteomic analysis of differences in the protein profiles of stems and leaves from two alfalfa genotypesqRT–PCR analysis of mRNA transcripts associated with randomly selected DEPs from stems (a) and leaves (b). FS: stem (F), MS: stem (M); FL: leaf (F), ML: leaf (M). The expression of each gene was calculated relative to the β-actin reference gene using the 2−△△CT method. Three biological replicates were analyzed, error bars denote SDs, and data were analyzed by one-way ANOVA (* p < 0.05, ** p < 0.01, Duncan’s multiple range test)Back to article page