Table 3 Stability of expression of the 20 candidate reference genes, as calculated by RankAggreg
Ranking | H. robusta -leaves | H. robusta -young stems | H. robusta -leaves & young stems | Different tissues | 4 °C treatment | MeJA treatment | PEG6000 treatment | All samples |
|---|---|---|---|---|---|---|---|---|
1 | PP2C59 | HIS1 | UBC17 | TUB-α | 60S-18 | UBC17 | HIS6 | TUB-α |
2 | UBC5B | ACT7 | UBC5B | PPIA95 | TUB-α | PPIA95 | MUB1 | PGK |
3 | HIS6 | TIP41 | ACT7 | TIP41 | PPIA95 | EF1 | PP2C57 | UBC5B |
4 | UBC17 | PGK | TUB-α | EF2 | ACT7 | HIS1 | UBC5B | UBC17 |
5 | HIS1 | PPIA95 | HIS1 | PP2C59 | PGK | TIP41 | EF1 | TIP41 |
6 | ACT7 | UBC5B | PPIA95 | UBC5B | PP2C57 | ACT7 | PGK | PP2C59 |
7 | TUB-α | MUB1 | HIS6 | HIS1 | UBC17 | HIS6 | EF2 | ACT7 |
8 | PPIA95 | EF2 | PP2C59 | ACT7 | EF2 | EF2 | SAMDC | PPIA95 |
9 | PGK | UBC17 | PGK | EF1 | SAMDC | UBC5B | ACT7 | HIS1 |
10 | EF2 | TUB-β | TIP41 | 60S-18 | UBC5B | PP2C57 | 60S-18 | EF2 |
11 | TIP41 | TUB-α | MUB1 | SAMDC | 60S-13 | PGK | PPIA95 | SAMDC |
12 | MUB1 | MUB1 | EF2 | UBC17 | HIS1 | TUB-β | PPIA26 | 60S-18 |
13 | PP2C57 | 60S-13 | 60S-13 | MUB1 | PP2C59 | 60S-18 | UBC17 | MUB1 |
14 | SAMDC | EF1 | 60S-18 | 60S-13 | 18S | TUB-α | PP2C59 | TUB-β |
15 | 60S-13 | PP2C59 | SAMDC | TUB-β | TUB-β | 60S-13 | 18S | HIS6 |
16 | 18S | 18S | EF1 | PGK | TIP41 | MUB1 | TUB-α | 18S |
17 | EF1 | HIS6 | TUB-β | 18S | EF1 | 18S | TIP41 | 60S-13 |
18 | 60S-18 | 60S-18 | 18S | PP2C57 | HIS6 | SAMDC | TUB-β | EF1 |
19 | TUB-β | PP2C57 | PP2C57 | HIS6 | MUB1 | PP2C59 | HIS1 | PP2C57 |
20 | PPIA26 | PPIA26 | PPIA26 | PPIA26 | PPIA26 | PPIA26 | 60S-13 | PPIA26 |