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Fig. 3 | BMC Plant Biology

Fig. 3

From: Pre-purification of diatom pigment protein complexes provides insight into the heterogeneity of FCP complexes

Fig. 3

77 K fluorescence spectra of the five AEC fractions. The spectra were normalized to the fluorescence emission maximum (Fig. 3a) or the excitation maximum of the Chl a fluorescence (Fig. 3b). For the 77 K fluorescence measurements the pigment protein complexes were adjusted to an optical density of 0.1 in the red part of the spectrum and then diluted with glycerol until a final glycerol concentration of 60% was obtained. Figure 3a shows the fluorescence emission spectra with a constant excitation at 440 nm, for the excitation spectra depicted in Fig. 3b the constant emission wavelength was set to the maximum of the emission spectrum. Fig. 3 shows typical emission and excitation spectra. For further details see the Methods section

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