Fig. 3From: Pre-purification of diatom pigment protein complexes provides insight into the heterogeneity of FCP complexes77 K fluorescence spectra of the five AEC fractions. The spectra were normalized to the fluorescence emission maximum (Fig. 3a) or the excitation maximum of the Chl a fluorescence (Fig. 3b). For the 77 K fluorescence measurements the pigment protein complexes were adjusted to an optical density of 0.1 in the red part of the spectrum and then diluted with glycerol until a final glycerol concentration of 60% was obtained. Figure 3a shows the fluorescence emission spectra with a constant excitation at 440 nm, for the excitation spectra depicted in Fig. 3b the constant emission wavelength was set to the maximum of the emission spectrum. Fig. 3 shows typical emission and excitation spectra. For further details see the Methods sectionBack to article page