Fig. 1

Elution profile of the pigment protein complexes of T. pseudonana separated by anion exchange chromatography (AEC). Figure 1 depicts the protein absorption at 280 nm and the stepwise increase of the KCl concentration. Before the separation, isolated thylakoids were solubilized with a β-DM per Chl ratio of 20. Solubilized thylakoids with a total amount of 200 to 500 μg Chl were loaded onto the AEC column. The numbers of the peaks denote the fractions that were collected and further characterized. Figure 1 shows a typical elution profile. For more information see the Methods section