Fig. 3

Mutant SlAGO1A transgenic transcripts were resistant to SlmiR168-mediated cleavage in tomatoes. a. Representation of the constructs used for transgenic expression of the SlmiR168-resistant mutant (rSlAGO1A) in tomatoes. Mutations were introduced at four locations (in red), and these base changes did not affect the native protein sequence. b. Amplification of the rSlAGO1 cDNA band located at 3100 bp. c. Amplification of the pri-SlmiR168a band at 159 bp