Fig. 5

Overexpression of TaWRKY42-B accelerates leaf senescence in Arabidopsis. a Phenotypic analysis of 6-week-old Col-0 and TaWRKY42-B-overexpressing plants. b Protein level of TaWRKY42-B in Col-0 and TaWRKY42-B-overexpressing plants. Ponceau S (P.S.) staining indicates equal loading. c Detached rosette leaves of 6-week-old Col-0 and transgenic plants were laid out. Chlorophyll content (d) and ion leakage rate (f) of the 6th and 7th detached leaves in (c). e DAB staining of 4-week-old Col-0 and TaWRKY42-B-overexpressing plants. g-j Detection of expression level of senescence-associated genes AtSAG12, AtSEN4, AtRBCS, and AtCAB1 in 4-week-old Col-0 and TaWRKY42-B-overexpressing plants by qRT-PCR. (Error bars indicate SD. Asterisks indicate significant differences. Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001)