Fig. 4

Silencing of TaWRKY42-B delays leaf senescence in wheat. a Phenotypic analysis of detached leaves of TaWRKY42-B-silenced plants and controls under darkness for 6 days. (DAT, Day after treatment). b Transcription level of TaWRKY42-B in leaves of TaWRKY42-B-silenced plants and controls. c DAB staining of detached leaves after dark treatment as shown in (a). d-e The expression level of TaWRKY42-B orthologs (TaWRKY42-A and TaWRKY42-D) in detached leaves of TaWRKY42-B-silenced plants and controls. Chlorophyll content (f) and ion leakage rate (g) of detached leaves as shown in (a). h-i Transcription level of senescence-associated genes TaSAG3, and TaSAG5 of detached leaves as shown in (a). (Error bars indicate SD. Asterisks indicate significant differences. Student’s t-test, *P < 0.05, **P < 0.01)