Fig. 4
Tissue specific expression profiles of BnNRT2 family genes as determined in RNA-seq and qRT-PCR assays. a Heat maps of relative expression from RNA-seq analysis of BnNRT2 expression in eight different tissues. PS: pistil, SM: stamen, OV: ovule, S: stem, L: leaf, R: root, SQ: silique, P: pericarp. b-d Summary of tissue specific expression of BnNRT2 genes determined in qRT-PCR assays. b Expression of BnNRT2s of different tissues in adult plants at different growth stages. Each tissue of adult plants were collected from field plots supplemented with N supplied as 180 kg/hm2 urea, in detail, junior leaf (JL), senior leaf (SL) and root (R) were collected at vegetative growth stage (namely over-wintering stage), flower (F) and stem (S) were collected at flowering stage, then seed (SD) and pericarp (P) were collected at reproductive growth stage. c The expression of BnNRT2.5 s in seedling tissues (21-days-old rapeseed seedlings). Rapeseed seedlings were reared in hydroponics cultures supplied with normal N treatment (9.5 mM N), L: leaf, R: root. d Expressions of BnNRT2.5 s and BnNRT2.7 s in the flower (F), stem (S), seed (SD) and pericarp (P) though qRT-PCR assays were further delineated in heat maps