Fig. 2

Gene structures and conserved domains in the NRT2 family protein of rapeseed. a A multiple sequence alignment of BnNRT2 family proteins constructed using DNAMAN software. The black frame and red frame represent the domain of MFS signature sequences and NNP signature sequences, respectively. b Amino acid sequences of the motif conserved across rapeseed NRT2s proteins, which contain signature sequences of MFS and NNP domains. The bigger the font size, the more likely the amino acid is at that location across all BnNRT2s proteins; c Boxes with different colors represent different conserved motifs, with the MFS and NNP motifs being denoted by circles and asterisks in the boxes, and a gene structure map of BnNRT2s was correspondingly constructed in GSDS2.0. Green boxes indicate exons, and black lines represent introns