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Table 5 Sequence analyses of CRISPR/Cas9-induced mutations at AhNFR5B DNA sequence site of transgenic AhNFR5B hairy roots

From: The application of CRISPR/Cas9 in hairy roots to explore the functions of AhNFR1 and AhNFR5 genes during peanut nodulation

  1. Sequence confirmation of nucleotide deletion and insertion mutations in peanut transgenic AhNFR5B samples. ‘WT’ represents wild type. Wild-type sequences are in black, difference between alleles is in blue, deletions are shown as dashes, and insertions are shown in red. Percentages next to sequences indicate the number of clones with sequence over the number of total clones sequenced. Δ is the base difference comparing with WT.