Skip to main content

Table 4 Mutation genotype and nodulation phenotype of independent AhNFR5-edited hairy root

From: The application of CRISPR/Cas9 in hairy roots to explore the functions of AhNFR1 and AhNFR5 genes during peanut nodulation

Samples Mutation at target 5AB1 site Mutation at target 5AB2 site GFP positivity Nodule phenotype
At Bt At Bt
F1 −5,-1, −3, −11 −4,-5,n −3,−7,-8,+ 1 +
F2 n,-2,-4,-1 n,-1,+ 1 n,-7,-8 n,-3,-7 + +
F3 -7,+ 1,-1 −5,+ 1 −2,n n,-7 +
F4 n n,-5 n n + +
F5 n,-5,+ 1 n,-4,-5 n n,−13 + +
F6 -1,-736 / n,-736 / +
F7 n n n n + +
F8 n,-1 n,-16 n n + +
F9 + 1,−34 -3,-4,+ 1 −5,n −2,n,-50 +
F10 n n n n + +
  1. ‘At’ represents target sequence in AhNFR5A gene; ‘Bt’ represents target sequence in AhNFR5B gene. ‘-’, ‘+’ and ‘n’ in the left four columns represent nucleotide deletions, insertions and no mutation respectively. ‘-’ and ‘+’ in the right two columns represent negative and positive