The application of CRISPR/Cas9 in hairy roots to explore the functions of AhNFR1 and AhNFR5 genes during peanut nodulation

Table 4 Mutation genotype and nodulation phenotype of independent AhNFR5-edited hairy root

Samples	Mutation at target 5AB1 site		Mutation at target 5AB2 site		GFP positivity	Nodule phenotype
Samples	At	Bt	At	Bt	GFP positivity	Nodule phenotype
F1	−5,-1,	−3, −11	−4,-5,n	−3,−7,-8,+ 1	+	–
F2	n,-2,-4,-1	n,-1,+ 1	n,-7,-8	n,-3,-7	+	+
F3	-7,+ 1,-1	−5,+ 1	−2,n	n,-7	+	–
F4	n	n,-5	n	n	+	+
F5	n,-5,+ 1	n,-4,-5	n	n,−13	+	+
F6	-1,-736	/	n,-736	/	+	–
F7	n	n	n	n	+	+
F8	n,-1	n,-16	n	n	+	+
F9	+ 1,−34	-3,-4,+ 1	−5,n	−2,n,-50	+	–
F10	n	n	n	n	+	+

‘At’ represents target sequence in AhNFR5A gene; ‘Bt’ represents target sequence in AhNFR5B gene. ‘-’, ‘+’ and ‘n’ in the left four columns represent nucleotide deletions, insertions and no mutation respectively. ‘-’ and ‘+’ in the right two columns represent negative and positive

ISSN: 1471-2229