Table 2 Target sequences of sgRNAs designed in this study
sgRNAs | Target sequence (5′-3′) | Targeted genes | Constructs (plasmid:sgRNAs) |
|---|---|---|---|
NFR1AB1 | TCTAGCTTCCTACTACATG | AhNFR1A2 and AhNFR1B2 | p201G/Cas9:NFR1AB1 + NFR1AB2 |
NFR1AB2 | TTACCGTTAACTGCTCCTG | ||
NFR5AB1 | GCAAGTAACATAAAGAATG | AhNFR5A and AhNFR5B | p201G/Cas9:NFR5AB1 + NFR5AB2 |
NFR5AB2 | CTTGGGGGCACAGTTTACA | ||
NFR5B | ACTCAACCCGAGCCTTCACA | AhNFR5B | p201G/Cas9:NFR5B |