Fig. 5

Validation of CRISPR/Cas9-induced mutations at both AhNFR1A2 and AhNFR1B2 DNA sequence sites. H1-L ~ H10-L: alignment of genomic sequences cloned from the long PCR products using DNA samples H1 ~ H10 of transgenic AhNFR1 hairy roots as templates. Target 1 and target 2 represent target NFR1AB1 and target NFR1AB2. Wild-type sequences are in black, deletions are shown as dots, and insertions are shown in blue. ‘-’, ‘+’ and ‘0’ on the right represent nucleotide deletions, insertions and no mutation, respectively