Fig. 5

RNP cleavage test in vitro and PCR-RE test after protoplast transformation. RNP cleavage test in vitro; t3-cleaved and t4-cleaved were used to cut the target 3 and target 4 PCR sequences by RNPs, while t3-uncleaved, as a control, was the sequence of target 3 PCR, and t4-uncleaved was the target 4 PCR sequence (a). ko-dig refers to Eco471 enzyme digestion of PCR amplification target sequences after RNP transformation of protoplasts; wt-dig refers to Eco471 enzyme digestion of target sequences of PCR amplification after blank transformation of protoplasts; wt-undig refers to PCR amplification after blank transformation of protoplasts, as a control. M indicates DNA marker (b)