Fig. 2

Experimental design and workflow of the transient genome editing system in banana protoplasts. The banana protoplasts were isolated from embryogenic cell suspension of Cavendish banana (AAA) (a), and then banana protoplasts were transformed by plasmids or RNPs through the PEG-mediated method (b). The genomic DNA of protoplasts was extracted 4 or 5 days after transformation. The mutation efficiency was measured by PCR-RE (c) or deep amplicon sequencing (d)