Fig. 8

a Genes in the plant organic acid pathway were regulated by NaHS treatment under iron deficiency and iron sufficiency conditions in roots of Glycine max plants. Transcript levels of genes are listed in the figure, and the detailed DEGs are listed in supplementary Table S8. Abbreviations of gene names are provided in supplementary Table S3. The relative mRNA levels of each gene were normalized to the mRNA of GmEIF1B and Gmactin. Data are presented as the mean ± SE (n = 3). b Effect of Fe deficiency and NaHS supply on the contents of citric acid and malic acid in leaves and roots of Glycine max plants. Error bars represents the mean ± SE. Columns labelled with different letters indicate significant differences at P < 0.05. A: –Fe, 1 μM Fe; B: –Fe + NaHS, seedlings treated with 100 μM NaHS and 1 μM Fe; C: +Fe, 50 μM Fe; D: +Fe + NaHS, seedlings treated with 100 μM NaHS and 50 μM Fe