Skip to main content

Table 1 Reduced transmission of the pigs-1 and gpi8–2 mutations through the male gametophyte

From: AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis

Female parent Male parent Observed No. of progeny TE (R/S) Χ2 P-value
BastaRa BastaSa
BastaR/+ Wild type 120 131 0.92 0.48 0.49
lre-7/+ Wild type 29 181 0.16 110 < 0.001
gpi8–2/+ Wild type 177 169 1.05 0.19 0.67
pigs-1/+ Wild type 255 268 0.95 0.32 0.57
Wild type BastaR/+ 189 193 0.98 0.04 0.84
Wild type gpi8–2/+ 0 607 0 607 < 0.001
Wild type pigs-1/+ 8 483 0.02 460 < 0.001
Wild type pigs-1/+; GFP-PIGS 108 142 0.76 4.62 0.03
  1. Wild type, Columbia-0 ecotype
  2. TE, Transmission efficiency was calculated as the quotient of the number of basta resistant (R) divided by basta susceptible (S) progeny of the indicated cross
  3. χ2 was calculated based on the expectation of a 1:1 segregation of basta resistance to susceptibility in the progeny. GFP-PIGS refers to the T2 line of pPIGS:GFP-PIGS #15–3
  4. aBasta resistant (BastaR) and Basta susceptible (BastaS) progeny. Basta resistance gene is linked with the T-DNA that is inserted into the AtGPI8 (gpi8–2) [25] and LRE (lre-7), AtPIGS (pigs-1), and in an unknown location in the genome of the BastaR/+ line [21]