A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow gun

Table 5 Efficiency of transgenic plant production after biolistic- mediated transformation of immature embryo-derived tissues of Timopheevi wheat (T.timopheevii Zhuk)

Callus induction/selection medium^a	No. IE explant cultured	Morphogenic callus formation (%)^b	No. bombarded explants	No. explants produced transgenic plants after dual GFP + PPT selection	Transformation efficiency (%)
Daminozide 0 mg/l	845	30.2a	255	0	0.0
Daminozide 12.5 mg/l	652	57.7b	376	2	0.5
Daminozide 25.0 mg/l	538	65.4b	352	0	0.0

^aMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l 2,4-D; 3 mg/l PPT was additionally added into selection medium
^bMeans having the same letter in the column has no significant differences according to Duncan’s multiple range test (P < 0.05)

ISSN: 1471-2229