A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow gun

Table 4 Summary of segregation analysis of GFP expression in T₁ progeny (embryos) and seeds (endosperms) of T₀ transgenic events of emmer wheat ‘Runo’ (T.dicoccum (Schrank)) ^a

Visual GFP expression segregation ratio (positive:negative)	Endosperms		Embryos		Events with the same segregation in embryos and endosperms
	No. independent T₀ events	Percentage	No. independent T₀ events	Percentage	No. independent T₀ events	Percentage
No. GFP expression	1	1	6	7	–	–
< 1:1	11	12	17	18	5	5
1:1	19	21	16	17	10	11
1:1–3:1	15	16	8	9	4	4
3:1	32	35	32	35	26	28
3:1–15:1	7	8	3	3	1	1
15:1	5	5	8	9	5	5
> 15:1	2	2	2	2	2	2
Total events with the same segregation in embryos and endosperm					53	58
Total No. events analysed	92		92		92

^aOnly events with normal fertility are presented, progeny were categorized into the respective classes by chi-square analysis (P > 0.05)

ISSN: 1471-2229