Table 4 Characterization and polymorphism levels of nine microsatellite loci in P. vietnamensis
Primers | Primer sequence (5′–3′) | Repeat motif | Fragment size (bp) | Ta (°C) | A | PIC | Ho | He | F | GenBank accession no |
|---|---|---|---|---|---|---|---|---|---|---|
L37 | F: GAGCGGGAGGGAGAGAGA R: CTTTCTCGTCGTCGTCATCA | (CATCAC)7 | 120–180 | 55 | 4 | 0.354 | 0.629 | 0.650 | 0.033 | MK802095 |
L39 | F: TTTGCCTCACTCCCCTGTAG R: AGAAGGAGGAGAGACCGAGG | (AGCGGC)5 | 176–201 | 55 | 3 | 0.332 | 0.439 | 0.511 | 0.146 | MK802096 |
L73 | F: TCTTGGGGATTGTGAAGGAG R: TTAAGGAACAGTGGCAGCAG | (TCTA)8 | 205–225 | 55 | 3 | 0.394 | 0.178 | 0.208 | 0.146 | MK802097 |
L111 | F: GCTCCACAACTCACTCCTCC R: TCTGTTCAGCTTCGTCCTCC | (TTC)11 | 197–230 | 55 | 4 | 0.325 | 0.723 | 0.610 | −0.185 | MK802098 |
L115 | F: CCCCATCATTCCATTGGTAG R: CTCAATCCCATCACGAGGAC | (TGT)10 | 221–239 | 55 | 3 | 0.442 | 0.386 | 0.628 | 0.386 | MK802099 |
L119 | F: CGTGTGTTACTGTTGTGGGG R: CGATTCTCACTCCCACCATT | (TGA)10 | 148–166 | 55 | 2 | 0.471 | 0.429 | 0.439 | 0.024 | MK802100 |
L139 | F: AATCATGTGGGACCGAAGAG R: TTGCATTTGGTTTTCTGTGC | (GAA)18 | 198–249 | 55 | 3 | 0.442 | 0.217 | 0.366 | 0.408 | MK802101 |
L145 | F: CCGTCTCCTTCAACTGCTTC R: AGTTGGGAATGAAGATTGCG | (CTT)15 | 247–265 | 55 | 2 | 0.493 | 0.231 | 0.371 | 0.379 | MK802102 |
L149 | F: CCTCCCCAAATCCTCCTCTA R: GACCTCTCCAGCTCCAACAG | (CTC)10 | 164–221 | 55 | 3 | 0.369 | 0.569 | 0.529 | 0.076 | MK802103 |