Fig. 6

Auxin-mediated stimulation of WOX and LBD gene expression in artificially fallen plants. The phylogenetic trees were generated using the Neighbor-Joining method of the MEGA7 software (https://www.megasoftware.net) (a, b). In the phylogenetic analysis, protein members that have been functionally characterized were marked in bold. For gene expression analysis, three-week-old plants grown in soil were sprayed with either 0.1 mM IAA or 1 μM NPA solution and then artificially fallen down to the soil surface (c, d). The first leaf nodes and their internodes were harvested at the indicated time points for the extraction of total RNA samples. Transcript levels were analyzed by RT-qPCR. Biological triplicates, each consisting of 15 plants, were statistically analyzed. Error bars indicate SE. a Phylogenetic analysis of Brachypodium WOX proteins. b Effects of auxin and NPA on the transcription of Brachypodium WOX genes. c Phylogenetic analysis of Brachypodium LBD proteins. d Effects of auxin and NPA on the transcription of LBD genes