Table 6 Description of the molecular markers applied in the study for the identification of the allelic state of Rht, VRN and Ppd genes

Rht-B1

Rht-B1p-WF:

5′ ACATGGCGGACGTGGTGC 3′

Rht-B1-R1:

5′ GCCGAGAGAGGACGAT 3’

Rht-B1a

425

[7, 66]

Rht-B1p-F:

5’ ACATGGCGGACGTGGTGT 3′

Rht-B1-R1:

5′ GCCGAGAGAGGACGAT 3’

Rht-B1p^a

(Rht17)

425

BF:

5’ GGTAGGGAGGCGAGAGGCGAG 3′

WR1:

CATCCCCATGGCCATCTCGAGCTG

Rht-B1a

237

[67]

BF:

5′ GGTAGGGAGGCGAGAGGCGAG 3′

MR1:

5′ CATCCCCATGGCCATCTCGAGCTA 3′

Rht-B1b^a

(Rht1)

237

BF:

5′ GGTAGGGAGGCGAGAGGCGAG 3′

W3:

5′ GGCCATCTCCAGCTGCTCCAGCTT 3’

Rht-B1a

228

[66]

BF:

5’ GGTAGGGAGGCGAGAGGCGAG 3′

M3:

5′ GGCCATCTCCAGCTGCTCCAGCTA 3’

Rht-B1e^a

(Rht11)

228

Rht-D1

DF2:

5’- GGCAAGCAAAAGCTTCGCG −3′

WR2:

5’- GGCCATCTCGAGCTGCAC −3’

Rht-D1a

264

[67]

DF:

5’- CGCGCAATTATTGGCCAGAGATAG − 3′

MR2:

5’- CCCCATGGCCATCTCGAGCTGCTA − 3’

Rht-D1b^a

(Rht2)

254

Ppd-D1

Ppd-D1_F:

ACGCCTCCCACTACACTG

Ppd-D1_R2:

CACTGGTGGTAGCTGAGATT

Ppd-D1_R1:

GTTGGTTCAAACAGAGAGC

Ppd-D1a

288

[68]

Ppd-D1b^a

414

VRN-A1

VRN1AF:

GAAAGGAAAAATTCTGCTCG

VRN1-INT1R:

GCAGGAAATCGAAATCGAAG

Vrn-A1a

965 + 876

[69, 70]

Vrn-A1b

714

VRN1AF:

GAAAGGAAAAATTCTGCTCG

VRN1-INT1R:

GCAGGAAATCGAAATCGAAG

vrn-A1^a

734

VRN-B1

Intr1/B/F:

CAAGTGGAACGGTTAGGACA

Intr1/B/R3:

CTCATGCCAAAAATTGAAGATGA

Vrn-B1a

709

[70]

Intr1:

ATCATCTTCTCCACCAAGGG

Intr1/B/R3:

CTCATGCCAAAAATTGAAGATGA

Vrn-B1a

Vrn-B1c

1124

737

[71]

Intr1/B/F:

CAAGTGGAACGGTTAGGACA

Intr1/B/R4:

CAAATGAAAAGGAATGAGAGCA

vrn-B1^a

1149

VRN-D1

Intr1/D/F:

GTTGTCTGCCTCATCAAATCC

Intr1/D/R3:

GGTCACTGGTGGTCTGTGC

Vrn-D1

1671

[70]

Intr1/D/F:

GTTGTCTGCCTCATCAAATCC

Intr1/D/R4:

AAATGAAAAGGAACGAGAGCG

vrn-D1^a

997

Rht-8

WMS261-F:

5’- CTCCCTGTACGCCTAAGGC −3′

WMS261-R:

5’- CTCGCGCTACTAGCCATTG − 3′

Rht-8c

192

[72]