Fig. 2

Map-based cloning and identification of sh1*. a The sh1* locus was mapped to a 97.12-kb interval between molecular ZM0064 and ZM0059 on chromosome 9. b Sequence alignment (partial) of SH1 in Z58 and sh1*. The dotted box marked the mutation site in sh1*. c Gene model of SH1. Gray lines represent introns and black boxes represent exons, gray box and arrow represent 5’UTR and 3’UTR, respectively. Mutation site of sh1* (arrow) and insertion site of uFmu-02854 (triangle) were indicated. d Heterozygous sh1* and uFmu-02854 were used for allelism test of sh1*. Hete: heterozygous, homo: homologous. Bar = 0.5 cm. e The sucrose synthase activity of the endosperm of 16 DAP kernels of sh1*, uFmu-02854, sh1*/uFmu-02854 as well as WT controls Z58 and W22. Values were means with SD. (***, P < 0.001, student’s t-test)