Fig. 5

Identification of interaction between CiMYB42 and promoters of CiSQS and CiOSC with yeast one-hybrid assays. a Co-transformation of empty pGADT7 and pAbAi-SQS under the condition of 200 ng/ml AbA; b Co-transformation of empty pGADT7 and pAbAi-OSC under the condition of 500 ng/ml AbA; c The interaction between CiMYB42 and CiSQS under the condition of 200 ng/ml AbA; d The interaction between CiMYB42 and CiOSC under the condition of 500 ng/ml AbA; (+) Positive control: pGADT7-p53 + Y1H [pAbAi-p53]; (−) Negative control: pGADT7 + Y1H [pAbAi-p53]; (1) pGADT7 + Y1H [pAbAi-SQS]; (2) pGADT7 + Y1H [pAbAi-OSC]; the recombinant Y1H Gold yeast strain were selected by SD/ -leu medium containing optimal AbA concentrations; 10–1, 10–2, 10–3 and 10–4 are different dilution ratio