Fig. 2

The molecular characteristics of HtMYB2. a The gene structure of HtMYB2. The black squares represent exons, and the black lines represent introns. b The phylogenetic tree of MYB proteins regulating anthocyanin biosynthesis. GbMYB2a:BAP47698.1; GbMYB1:BAJ17661.1; GhMYB10:AAK19615.1; HaMYB113:XP_022033329.1; AmROSEA2:ABB83827.1; LjMYB89:AFK35838.1; LrAN2:QCS14086.1; CaAN2:NP_001311547.1; PhAN2:AB982128.1; SlAN2:FJ705319.1; VvMYBA1:ABD72954.1; VvMYBA2:BAD18978.1; AmROSEA1:ABB83826.1; AmVENOSA:ABB83828.1; AtMYB113:NM_105308.2; AtMYB114:NM_001334235.1; AtMYB75: ABB03879.1; AtMYB90:NP_176813.1; LjMYB3:ALU11262.1; LjMYB4:ALU11258.1; AmMYB308:ABI26190.1; AmMYB315:AAV70655.1; AtMYB4:NP_195574.1; AtMYB32:NP_195225.1; AtMYB7:OAP08362.1; AtMYB6:NP_192684.1; AtMYB8:BAE99960.1; AtMYB3:NP_564176.2; ZmMYBP:AAL90641.1; LjMYB6:E5L8F7.1; AtMYB11:NP_191820.1; AtMYB12:ABB03913.1; AtMYB111:EFH41988.1; LjMYB7:AKV17427.1; AtMYB5:NP_187963.1; LjMYB63:ALU11252.1; BJ185183:XP_024388078.1; DcMYB1:XP_017224603.1; NtMYB2:XP_009629052.1; LjMYB1:AFK36130.1; LjMYB49:ALU11251.1; LjMYB60:XP_019055104.1; OsMYB4:XP_015633465.1; AtMYB15:NP_188966.1; AtMYB13:NP_172108.1; AtMYB14:XP_002881162.1; LjMYB12:3760052; LjMYB90:ALU11257.1; TaMYBA6:BAH36890.1; LjMYB71:ALU11256.1; AtMYB123:ABK28720.1; LjMYB38:BAG12893.1; LjMYB41:BAG12894.2; LjMYB72:BAG12893.1; AtMYB66:NP_196979.1; LjMYB88:ALU11254.1; AtMYB0:AAL01241.1;AtMYB23:CDY44571.1; ZmMYC1:1613412E; AtMYB82:AAF14064.1; CmMYB6:AKP06190.1; HaMYB90:XP_022033410.1; c The amino acid alignment of HtMYB2 and the closest anthocyanin-related MYB transcription factors. CmMYB6: AKP06190.1; GbMYB1: BAJ17661.1; GbMYB2a: BAP47698.1; GhMYB10: AAK19615.1; HaMYB90: XP_022033410.1. The triangle repesent the site of the different amino acid of HtMYB2 from QY1 and QY3. The amino acid “R” in QY1 was “K” in QY3