BMC Plant Biology

Table 1 Parameters of producing doubled haploids with F₁s and selected sterile plants of F₂ and F₃

From: Development of thermo-photo sensitive genic male sterile lines in wheat using doubled haploid breeding

Year	Combinations	Statistics in producing embryos, haploid seedlings and DH lines^*
Year	Combinations	NS	NC	NE	NHS	NDH	RE (%)	RHS (%)	RCD (%)
2014/2015	K78S/14Y₆–686	25	756	136	84	76	17.99^a	61.76^c	90.48^cde
2014/2015	K456S/14Y₆–686	15	510	214	146	110	41.96^d	68.22^d	75.34^a
2014/2015	K78S/14Y₆–438	32	928	362	152	130	39.01^c	41.99^a	85.53^bc
2014/2015	K456S/14Y₆–438	24	654	334	180	148	51.07^g	53.89^b	82.22^b
2015/2016	K78S/14Y₆–172	16	564	152	110	102	26.99^b	72.37^e	92.73^e
2015/2016	K456S/14Y₆–172	18	532	234	147	128	43.98^e	62.82^c	87.07^bcd
2015/2016	K456S/14Y₆–23	11	360	173	130	119	48.06^f	75.14^e	91.54^de
2016/2017	K456S/14Y₇–484	40	1292	323	210	107	25.00^b	65.02^cd	̶^**
Total / average		181	5596	1928	1159	920	36.76	62.65	86.42

^*NS No. of pollinated spikes, NC No. of caryopses, NE No. of embryos obtained, NHS No. of haploid seedlings germinated from embryos, NDH No. of DHs, RE Rate of embryo (NE·NC^− 1), RHS Rate of haploid seedling (NHS·NE^− 1), RCD Rate of chromosome doubling (NDH·NHS^− 1)
^**Part of colchicine treated plants was damaged by frost. Different letters in the last three rows mean significant at 0.05 level

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