Fig. 7

Bimolecular fluorescence complementation validates the bio-interaction of OsNIP2;1 and ATP-synβ. Lsi1 was infused with the N-terminal domain of YFP and ATP synthase subunit β and OsSHMT was infused with the C-terminal domain of YFP. Lsi1 with N-terminal domain was then co-expressed with ATP synthase subunit β or OsSHMT with the C-terminal domain of YFP, respectively, in the leaves of Nicotiana benthamiana for 48 h, and YFP fluorescence was detected using laser confocal microscopy under 488-nm excitation light. The subcellular localisation of Lsi1 was also detected in the leaves of Nicotiana benthamiana