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Table 1 Fatty acid composition (mol%) of tobacco seeds from wild type and fad2–2 mutant plants

From: Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9-mediated knockout of NtFAD2–2

  CK Low temperature treatment
WT fad2–2#06 fad2–2#16 WT fad2–2#06 fad2–2#16
16:0 12.1 ± 0.9 8.6 ± 0.7 8.5 ± 0.6 10.2 ± 0.8 8.3 ± 0.8 8.5 ± 0.9
18:0 3.3 ± 0.5 4.1 ± 0.3 3.7 ± 0.1 3.1 ± 0.4 3.9 ± 0.5 3.4 ± 0.1
18:1Δ9 11.6 ± 0.7 79.6 ± 3.6 78.5 ± 2.8 9.1 ± 0.5 78.6 ± 4.2 77.9 ± 3.7
18:2Δ9, 12 72.4 ± 3.1 6.8 ± 0.2 8.4 ± 0.6 76.7 ± 4.3 7.2 ± 0.4 9.1 ± 0.8
18:3Δ9, 12, 15 0.6 ± 0.06 0.9 ± 0.08 0.8 ± 0.06 0.9 ± 0.08 1.0 ± 0.1 0.9 ± 0.1
  1. 16:0, palmitic acid; 18:0, stearic acid; 18:1Δ9, oleic acid; 18:2Δ9, 12, linoleic acid; 18:2Δ9, 12, 15, linolenic acid. The data are the mean ± SD of three independent experiments