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Table 1 Fatty acid composition (mol%) of tobacco seeds from wild type and fad2–2 mutant plants

From: Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9-mediated knockout of NtFAD2–2

 

CK

Low temperature treatment

WT

fad2–2#06

fad2–2#16

WT

fad2–2#06

fad2–2#16

16:0

12.1 ± 0.9

8.6 ± 0.7

8.5 ± 0.6

10.2 ± 0.8

8.3 ± 0.8

8.5 ± 0.9

18:0

3.3 ± 0.5

4.1 ± 0.3

3.7 ± 0.1

3.1 ± 0.4

3.9 ± 0.5

3.4 ± 0.1

18:1Δ9

11.6 ± 0.7

79.6 ± 3.6

78.5 ± 2.8

9.1 ± 0.5

78.6 ± 4.2

77.9 ± 3.7

18:2Δ9, 12

72.4 ± 3.1

6.8 ± 0.2

8.4 ± 0.6

76.7 ± 4.3

7.2 ± 0.4

9.1 ± 0.8

18:3Δ9, 12, 15

0.6 ± 0.06

0.9 ± 0.08

0.8 ± 0.06

0.9 ± 0.08

1.0 ± 0.1

0.9 ± 0.1

  1. 16:0, palmitic acid; 18:0, stearic acid; 18:1Δ9, oleic acid; 18:2Δ9, 12, linoleic acid; 18:2Δ9, 12, 15, linolenic acid. The data are the mean ± SD of three independent experiments