Skip to main content

Table 3 Cosegregation of RYMV resistance and allelic state on CPR5–1

From: Allele mining unlocks the identification of RYMV resistance genes and alleles in African cultivated rice

 

Phenotype and genotype

F2 population

Total

rymv2-Rx

Htz

Rymv2-S1

Og133 (rymv2-R2) x Og431 (Rymv2-S1)

11 R, 50S (p = 0,23)

10 R

22 S

3 S

Og133 (rymv2-R2) x Tog7291 (rymv2-R1)

55 R, 1S

   

Og183 (rymv2-R3) x Og82 (Rymv2-S1)

14 R, 36 S (p = 0,62)

14 R

26 S

10 S

Og213 (rymv2-R4) x Og82 (Rymv2-S1)

13 R, 42 S (p = 1)

13 R

31 S

11 S

Og213 (rymv2-R4) x Tog7291 (rymv2-R1)

100 R

   

Og491 (rymv2-R5) x Og431 (Rymv2-S1)

24 R, 47 S (p = 0,10)

21 R

3 R, 30 S

17 S

Og491 (rymv2-R5) x Tog7291 (rymv2-R1)

56 R

   

Og256 (rymv2-R6) x Og82 (Rymv2-S1)

18 R, 52 S (p = 0,89)

18 R, 2S

37 S

13 S

Og256 (rymv2-R6) x Tog7291 (rymv2-R1)

45 R

   
  1. F2 plants were evaluated for RYMV resistance based on symptom observations. The phenotype is indicated with R for resistant plants and S for susceptible ones. For populations derived from crosses with Og82 and Og431 susceptible accessions, the observed phenotypic segregation was compared to a 1R/3S segregation ratio, based on a binomial exact test. For these populations, genotyping on the CPR5–1 gene was generally performed on all plants with CAPS or dCAPS markers, except for the (Og133 x Og431) population for which genotyping was based on Sanger sequencing and only performed on a subset of 35 plants. The genotype is indicated as “rymv2-Rx” for plants homozygous for alleles rymv2-R2 to -R6, “Rymv1-S1” for plants homozygous for the Rymv1-S1 allele, and Htz for heterozygous plants. F2 plants derived from crosses with Tog7291 were not genotyped