Fig. 5

phasiRNA target gene expression profile. a: Heat map displaying the expression pattern of the predicted phasiRNA targets in the cotton genome. PhasiRNAs were derived from GhMYB2 loci. Only conserved tasiRNAs that were consistently expressed in ovule, anther, hypocotyl tissues were selected. The phasiRNA targets were clustered according to their tissue expression patterns. b: Venn diagram illustrating differences between the expression patterns of the predicted phasiRNA target genes. All comparisons were against to the early ovule tissues. c, Degradome analysis of the down-regulated phasiRNA targets in anther, hypocotyl and EC tissues. Box plot showing the abundance of degraded fragments in anther, hypocotyl and EC tissues using RPKM values. The central line for each box plot indicates the median. The yellow represents the population of down-regulated phasiRNA targets in anther and hypocotyls tissue. The total levels of degraded fragments of coding genes were used as a background control (green). The P value was calculated using the Wilcoxon rank sum test