Fig. 4

GUS staining and PCR screening of transgenic and non-transgenic hairy roots. a GUS staining assay from a composite plant. b Validation of GUS-positive roots using PCR. Lanes 1–10, GUS-positive root; Lane 11, K599–1305. Lane 12, GUS-negative root. c Amplification of T-DNA flanking sequences by TAIL-PCR. The secondary (Lane 1, 3 and 5) and tertiary TAIL-PCR product (Lane 2, 4 and 6) are shown from independent transgenic roots (Lanes 1–2, 3–4, and 5–6 are from line 1, 2 and 3, respectively)