Fig. 3

Protein sequence comparisons of NtAQP subfamilies. a Diagrammatic illustration of an AQP depicting protein topology and lengths of the; N-terminal tail (N-term), TransMembrane domains (TM) 1–6, Loops A-E, and C-terminal tail (C-term). The average amino acid (aa) lengths of each structural feature are listed for the different NtAQP subfamilies. Common length of a domain is represented in grey, while deviations from the common length are in colour; PIPs (orange), NIPs (purple), SIPs (green), TIPs (blue) and XIPs (yellow). b Overall and intra-domain sequence similarities for each NtAQP sub-family. Schematic representation of the AQP domains is illustrated at the top, with aligned columns showing protein sequence identical sites (black) and the BLSM62 similarity score (grey) between members of the given NtAQP subfamily