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Table 2 Effect of different combination of soil-water (W), nitrogen (N) and phosphorus (P) on the levels of chlorophyll a (Chl a), chlorophyll b (Chl b), total chlorophylls (Total Chls), malondialdehyde (MDA) and proline (Pro), and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the leaves of A. fruticosa

From: Improvement of growth performance of Amorpha fruticosa under contrasting regime of water and fertilizer in coal-contaminated spoils using response surface methodology

No. of Trt

Level of treatments

Chl a

(mg g− 1 FW)

Chl b

(mg g− 1 FW)

Total Chls

(mg g− 1 FW)

MDA

(μmol g− 1 FW)

Pro

(μmol g− 1 FW)

SOD

(U g− 1 FW)

CAT

(U min− 1 g− 1 FW)

POD

(U min− 1 g− 1 FW)

W (% FC)

N (mg kg− 1)

P (mg kg− 1)

1

48.1

24.32

36.49

2.38 ± 0.04e

1.49 ± 0.04fg

3.87 ± 0.03gh

35.23 ± 4.59ab

3.96 ± 0.03c

139.56 ± 6.98abc

193.21 ± 3.93ab

213.74 ± 5.58b

2

71.9

24.32

36.49

2.54 ± 0.01b

1.78 ± 0.06cd

4.32 ± 0.05de

33.42 ± 4.12abcd

2.12 ± 0.03l

117.45 ± 8.12efgh

182.34 ± 3.09bcd

132.75 ± 5.38k

3

48.1

95.68

36.49

2.41 ± 0.07de

1.68 ± 0.06de

4.09 ± 0.04fg

30.74 ± 2.2abcde

5.15 ± 0.04a

142.31 ± 7.27ab

187.78 ± 7.17abc

161.21 ± 7.14ghi

4

71.9

95.68

36.49

2.52 ± 0.02bcd

1.77 ± 0.07cd

4.29 ± 0.05ef

23.34 ± 2.07e

2.56 ± 0.03j

107.38 ± 4.28hijk

167.48 ± 4.2efgh

187.03 ± 7.85cdef

5

48.1

24.32

143.51

2.31 ± 0.05e

1.32 ± 0.03g

3.63 ± 0.07i

31.15 ± 4.63abcde

2.32 ± 0.04k

135.87 ± 10.5bcd

173.28 ± 3.43def

254.66 ± 8.43a

6

71.9

24.32

143.51

2.42 ± 0.04cde

1.4 ± 0.05g

3.82 ± 0.09hi

26.01 ± 2.26de

2.93 ± 0.04hi

133.24 ± 3.31bcde

152.97 ± 3.61i

149.42 ± 6.28ijk

7

48.1

95.68

143.51

2.59 ± 0.02ab

1.97 ± 0.08ab

4.56 ± 0.1abc

36.74 ± 2.53a

3.04 ± 0.03h

129.13 ± 5.15bcdefg

192.28 ± 5.01ab

143.77 ± 5.5jk

8

71.9

95.68

143.51

2.61 ± 0.01ab

2.02 ± 0.06a

4.63 ± 0.08ab

26.43 ± 2.65cde

2.82 ± 0.05i

113.4 ± 5.92ghij

168.53 ± 3.27efgh

155.45 ± 7.4hij

9

40

60

90

2.53 ± 0.04bc

1.84 ± 0.03bcd

4.37 ± 0.06cde

34.41 ± 5.05abc

2.88 ± 0.03i

154.68 ± 9.42a

196.15 ± 3.95a

212.68 ± 7.38b

10

80

60

90

2.69 ± 0.04a

2 ± 0.04ab

4.69 ± 0.04a

25.81 ± 0.94de

1.54 ± 0.03m

109.63 ± 3.68hijk

171.05 ± 3.79efg

148.88 ± 5.32ijk

11

60

0

90

2.16 ± 0.07f

1.06 ± 0.04h

3.22 ± 0.11j

31.82 ± 2.17abcd

4.27 ± 0.06b

131.36 ± 7.31bcdef

167.59 ± 2.3efgh

201.24 ± 3.31bc

12

60

120

90

2.36 ± 0.03e

1.59 ± 0.03ef

3.95 ± 0.06gh

28.48 ± 1.37abcde

4.15 ± 0.04b

115.05 ± 4.46fghi

178.51 ± 5.79cde

148.32 ± 4.26ijk

13

60

60

0

2.53 ± 0.05bc

1.86 ± 0.03abc

4.39 ± 0.08cde

31.3 ± 3.52abcde

3.62 ± 0.02ef

118.6 ± 7.85defgh

184.76 ± 2.17bc

169.93 ± 7.67fgh

14

60

60

180

2.55 ± 0.04b

1.87 ± 0.02abc

4.42 ± 0.06bcde

29.28 ± 1.3abcde

2.37 ± 0.06k

124.27 ± 7.16cdefgh

158.86 ± 3.24hi

174.63 ± 4.77efg

15

60

60

90

2.57 ± 0.02b

1.96 ± 0.07ab

4.53 ± 0.09abcd

29.34 ± 2.58abcde

3.73 ± 0.06de

96.16 ± 4.09jkl

164.34 ± 3.6fgh

191.82 ± 3.7cde

16

60

60

90

2.56 ± 0.02b

1.95 ± 0.02ab

4.51 ± 0.02abcde

28.15 ± 1.82bcde

3.81 ± 0.06d

94.32 ± 3.03kl

166.73 ± 2.55fgh

187.03 ± 7.03cdef

17

60

60

90

2.58 ± 0.01ab

1.94 ± 0.08abc

4.52 ± 0.09abcd

29.56 ± 3.68abcde

3.62 ± 0.04ef

97.96 ± 3.78ijkl

161.39 ± 3.74ghi

183.31 ± 5.45def

18

60

60

90

2.55 ± 0.02b

1.94 ± 0.1abc

4.49 ± 0.11abcde

32.33 ± 1.87abcd

3.49 ± 0.04fg

89.22 ± 3.98l

168.92 ± 2.56efgh

189.53 ± 6.54cde

19

60

60

90

2.56 ± 0.03b

1.98 ± 0.07ab

4.54 ± 0.09abcd

31.06 ± 2.14abcde

3.85 ± 0.03cd

95.6 ± 2.35kl

171.49 ± 3.74defg

193.15 ± 4.88cd

20

60

60

90

2.54 ± 0.02b

1.92 ± 0.08abc

4.46 ± 0.09abcde

31.15 ± 2.8abcde

3.43 ± 0.05g

93.27 ± 6.51kl

165.97 ± 3.65fgh

185.38 ± 5.5cdef

  1. Values are means ± standard errors (n = 4). Different alphabetical letters (a, b, c, etc) within the same column indicate significant differences among various treatments according to a least significant difference test (LSD) (P <  0.05). Trt treatment, FC field capacity