Fig. 4

JA biosynthesis gene expression, JA content and JAZ1 degradation analyses. The mature leaves of poplar plants grown in greenhouse were treated using leaf-disc method for various time intervals and then used for RNA extraction. a qRT-PCR analyses of JA biosynthesis genes in the wounded leaves. PtrAOC2–3, a putative allene oxide cyclase gene in Populus; PtrOPR3–1, a putative OPDA reductase 3 gene in Populus; PtrACX1–2, a putative acyl-CoA oxidase gene in Populus; PtrJAR1–1, a putative JASMONATE RESISTANT gene (a JA-Ile biosynthesis gene) in Populus. b JA and JA-Ile content in the wounded leaves of poplar plants grown in greenhouse. The leaves treated for various time intervals were used to be tested. c qRT-PCR analyses of PtrJAZ1 expression in the wounded leaves. d Western blotting analyses of JAZ1 protein in the wounded leaves. Total proteins were extracted from the leaf-discs treated for various time intervals. An amount of 30 μg proteins were separated by 10% SDS-PAGE and hybridized with Arabidopsis JAZ1 antibodies (JAZ1) or plant actin antibodies (Actin), respectively. Each gene expression in the untreated leaves (0 h) was set to 1. Three independent replicates of measurements were performed for each time point, and the values are means and standard deviations (n = 3). * and ***, significant differences at P < 0.05 and P < 0.001 (Student’s t-test). The original uncropped blot image was shown in additional file 6: Fig. S6