Fig. 5From: Ultrahigh-activity immune inducer from Endophytic Fungi induces tobacco resistance to virus by SA pathway and RNA silencingTranscriptome analysis after ZNC treatment in Nicotiana. Benthamiana. RNA samples were taken from five or six leaf stage leaves, which were treated with 0 (H2O) or 150 ng/mL of ZNC for 2 h. Each treatment contain three samples (plants). a A set of comparison heatmap for ZNC treatment at 2 hpi. The red color in the figure indicates the high-expression genes, and the blue color indicates the low-expression genes. b Volcano plot showing fold change and adjusted p-value of normalized read counts of the transcriptome sequencing data. The criteria of log2| (fold change) | ≥ 1 and padj ≤0.05 were used to identify the DEGs. Green dots indicate the downregulated DEGs (1983 genes), and red dots indicate the upregulated DEGs (2801 genes). c GO enrichment top 10 (upregulated) term of biological process. The numbers of -log10P value of differential genes between H2O and ZNC groups in every term > 2 were screened, and the top 10 terms were sorted by the -log10P value corresponding to each term. d Partial KEGG pathway classification. Abscissa axis is the ratio of DEG in a pathway: all DEG in KEGG level 2 pathway (%), ordinate axis is the name of pathway. The numbers above the column represent the quantity of DEG in the pathway.Back to article page