Fig. 2

IbSUT4 localized to the tonoplast and plasma membrane. a, b Protoplasts expressing different GFP-fusion constructs. c Protoplasts expressing the IbSUT4-GFP-fusion construct incubated with 50 μg mL^− 1 PI in W5 buffer (pH = 5.7) for 30 min. GFP is indicated in green, and PI fluorescence and chlorophyll autofluorescence are indicated in red. A. Overview image of the tobacco protoplasts transformed with p35S-GFP. B. Overview image of tobacco protoplasts transformed with p35S:IbSUT4-GFP. C. Protoplasts expressing p35S:IbSUT4-GFP with PI in the nucleus. Scale bar =10 μm