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Fig. 8 | BMC Plant Biology

Fig. 8

From: Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Fig. 8

Effect stomatal aperture and gene expression for gma-miR398c knockout and overexpression in composite soybean plants. a Analysis of stomatal aperture of leaves before and after drought stress for soybean plants. The second tri-foliate from the top were chosen for stoma observation. Data are average of 30 views ± SD, asterisks indicate significant difference applying ANOVA between drought treated K599 and transgenic plants (*, P < 0.05; **, P < 0.01; ***, P < 0.001). Bar = 10 μm. b Analysis of miR398c gene expression in K599, OE-miR398c, KO-1 and KO-2 transgenic soybean hairy roots. c Analysis of GmCSD1a/b, GmCSD2a/b/c and GmCCS expression in K599, OE-miR398c, KO-1 and KO-2 transgenic soybean hairy roots. Data are means of ten biological replicates ± SD (10 plants per replicate)

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BMC Plant Biology

ISSN: 1471-2229

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