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Fig. 6 | BMC Plant Biology

Fig. 6

From: Overexpression of an apple LysM-containing protein gene, MdCERK1–2, confers improved resistance to the pathogenic fungus, Alternaria alternata, in Nicotiana benthamiana

Fig. 6

ROS accumulation and callose deposition in Nb leaves responding to A. alternata infection. a ROS accumulation was visualized by DAB staining and (c) quantified by xylenol orange assay. b Callose deposition was visualized by aniline blue staining and (d) quantified by dot counting using Image J software based on 9 photographs. e H2O2 generation in response to chitin treatment using luminol-based assays. The data were analyzed for statistical differences by a two-way ANOVA followed by Tukey’s post-hoc test. Bars indicate the mean ± SD of 5 independent biological replicates for H2O2 assays and 9 replicates for callose assays. Asterisks indicate significant differences when compared to the WT at the indicated time points (** p < 0.01)

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