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Fig. 2 | BMC Plant Biology

Fig. 2

From: Overexpression of an apple LysM-containing protein gene, MdCERK1–2, confers improved resistance to the pathogenic fungus, Alternaria alternata, in Nicotiana benthamiana

Fig. 2

Subcellular localization and ligand binding of the MdCERK1–2 protein. a Subcellular localization of MdCERK1–2. The MdCERK1–2-GFP fusion gene was transiently expressed in Nb leaves using the A. tumefaciens-mediated method and observed with a confocal microscope (bottom). The control expressing GFP was also observed (top). b Membrane proteins from Nb plants expressing MdCERK1–2-GFP were prepared and separated with SDS-PAGE. The presence of MdCERK1–2-GFP in membrane proteins was determined by immunobloting with an anti-GFP antibody. S, soluble protein; T, total protein; M, membrane protein; CBB, Coomassie brilliant blue staining. Anti-UGPase was used as internal reference of cytoplasm protein. c MdCERK1–2-ECD binds to chitin, but does not bind to PGN. Binding proteins were separated by SDS-PAGE and detected by immunobloting with an anti-His antibody

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