Fig. 2

Tissue specific expression analysis and subcellular localization of GmNFYA5. (A) Expression abundance of the GmNFYA5 gene in soybean tissues at the seedling and flowering stages. Soybean tissues under normal conditions were used to extract total RNA. The relative transcript levels of GmNFYA5 in soybean tissues are indicated by vertical columns. GmNFYA5 transcript in roots was set at 1.0, and the internal control was GmCYP2. SD for three biological replicates is represented by error bars. Significant differences at P < 0.05 are indicated by different letters above the columns. (B) Expression pattern of GmNFYA5 promoter: GUS in various tissues of transgenic Arabidopsis plants. a 5-day-old seedling, b rosette leaf, c cauline leaf, d root, e flower, f silique. Scale bar, 2 mm (C) Subcellular localization of GmNFYA5. Fluorescence of GFP-GmNFYA5 and mCherry-GmNFYA3 fusion proteins in transformed cells was localized exclusively to the nucleus. Scale bar, 10 μm