Fig. 1

GmNFYA5 expression as affected by drought stress and ABA. (A) Transcripts of 21 NF-YA genes were analyzed using qRT-PCR in soybean under drought stress. Expression levels were normalized to that of GmCYP2. GmNFYA5 transcript at 0 h was set at 1.0, and SD for three biological replicates is represented by error bars. (B) Transcript of GmNFYA5 analyzed in response to ABA treatment in soybean. Expression levels were normalized to that of GmCYP2. GmNFYA5 transcript at 0 h was set at 1.0, and SD for three biological replicates is represented by error bars. (C) qRT-PCR of GmNFYA5 transcript in soybean plants in response to drought stress with the treatment of 1 mM NAP. Control, drought and drought + NAP are indicated by CTR, D and D + NAP, respectively. The level of GmNFYA5 transcript under control conditions was set at 1.0, and the internal control was GmCYP2. SD for three biological replicates is represented by error bars. Significant differences at P < 0.05 are indicated by different letters above the columns