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Fig. 8 | BMC Plant Biology

Fig. 8

From: Comparative transcriptome analysis indicates that a core transcriptional network mediates isonuclear alloplasmic male sterility in wheat (Triticum aestivum L.)

Fig. 8

Quantification of physiological indices and histological analysis. a Total soluble sugar contents of anthers in the binucleate stage. b ATP content of anthers in the binucleate stage. c O2 contents. d H2O2 contents. Student’s t-test *p < 0.05 **p < 0.01. Each value represents means ± SD (n = 3). e TUNEL assays to detect anther tapetum PCD. Scale bars represent 50 μm. f Transmission electron micrographs of the tapetum. Scale bars represent 2 μm. g Transmission electron micrographs of microspore. Scale bars represent 2 μm. Luns, late uninucleate stage; Bns, binucleate stage; E: epidermis; En: endothecium; T: tapetum; Tds: tetrads; Msp: microspores; Ta: tapetosome; PE, primexine; Se, sexine; DM, deposited materials; PM, plasma membrane; In, intine

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