The in vivo impact of MsLAC1, a Miscanthus laccase isoform, on lignification and lignin composition contrasts with its in vitro substrate preference

Table 2 Absolute contents of H, G, and S monomers in lignin of different Arabidopsis lines

Arabidopsis Line	H lignin	G lignin	S lignin	Total	Relative yield/%
lac4–2 lac17	2.3 ± 0.9	60.3 ± 5.6 ^a	47.8 ± 1.1 ^a	110.4 ± 7.6 ^a	68.2 ± 0.2 ^a
pAtLAC17::Dummy	1.7 ± 0.2	59.3 ± 0.4 ^a	46.0 ± 2.7 ^a	107.0 ± 2.6 ^a	66.1 ± 0.1 ^a
pAtLAC17::MsLAC1	2.3 ± 0.2	113.5 ± 6.2 ^b	70.6 ± 6.4 ^b	186.3 ± 10.4 ^b	93.4 ± 0.2 ^c
Col-0	1.8 ± 0.6	149.2 ± 30.2 ^b	68.9 ± 8.0 ^b	219.8 ± 38.6 ^b	100 ^b
p35S::Dummy	2.2 ± 0.2	150.8 ± 3.5 ^b	58.9 ± 1.3 ^ab	211.9 ± 4.8 ^b	105.1 ± 0.1 ^b
p35S::MsLAC1	3.2 ± 1.7	192.1 ± 16.3 ^c	76.6 ± 5.2 ^b	271.8 ± 20.2 ^c	111.1 ± 0.2 ^d

Yields of H, G and S monomers after thioacidolysis released from extract-free samples (i.e. after acetone extraction) are presented as μmol per gram sample. Relative yields are calculated based on total amount of monomer per gram of Klason lignin. Wild type (Col-0) was used as control and its yield arbitrarily set to 100%. All measurements were repeated three times. One-way ANOVA (followed by Tukey test) was used to determine significant differences at P < 0.05. Samples with significant statistic difference are labelled with different letters

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