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Fig. 5 | BMC Plant Biology

Fig. 5

From: A T-DNA mutant screen that combines high-throughput phenotyping with the efficient identification of mutated genes by targeted genome sequencing

Fig. 5

Re-screen of candidate mutants. Ten mutants defective in genes identified by the target enrichment approach were re-screened. The genes AT1G13860 (QUL1) and AT5G55620 were also identified by adapter ligation-mediated PCR. The tested mutants were not genotyped. Ion leakage was determined upon fumigation with 30 ppm NO2 whereas stomatal conductance was measured in untreated plants. Asterisks indicate statistically significant differences (p ≤ 0.05) between WT and tested mutants according to one-way ANOVA and Holm-Sidak posthoc test (ion leakage: n = 15–28; stomatal conductance: n = 12–23)

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BMC Plant Biology

ISSN: 1471-2229

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