Fig. 4

Representative images of aniline blue-stained stigmas from the bkn1 bkn2 mutants. a-d. Aniline blue-stained pistils, following 2 h pollination with Col-0 pollen. Brightfield images (left) show pollen grains adhered to the stigmatic papillae, and fluorescent images (right) show aniline blue-stained pollen tubes (a), bkn1–3 bkn2–3 (b), bkn1–3 bkn2–4 (c) and bkn1–3 bkn2–5 (d). All show similar levels of pollen adhesion and pollen tube growth. Scale bar = 100 μm