Fig. 4

Differentially expressed genes (DEGs) in MIR7695-Ac mutant plants relative to WT-Az plants by RNA-seq analysis. Leaves of three-week-old plants were used (a) Number of DEGs and Gene Ontology (GO) analysis of DEG function. Up-regulated genes (log2 fold change [FC] ≥ 1; purple) and down-regulated genes (log2FC ≤ − 1; green) genes (p < 0.05, false discovery rate [FDR] < 0.05, n = 2). Pie charts represent the five general GO terms enriched in up- and downregulated DEGs. b Enriched terms in the “binding” and “catalytic activity” categories. c Heatmaps showing RNAseq expression level (left panel; log10 [FPKM+ 1]) and FC (right panel; log2FC) of DEGs. Gene expression is represented from pale yellow (less expressed) to blue (more expressed). Upregulated (log2FC ≥ 1; purple) and downregulated (log2FC ≤ − 1; green) DEGs. Data are means (n = 2). The full gene names and ID list are in Additional file 8: Table S5